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Course Outline
Lecture Notes
Readings
Assay Manual
Experiments
Assays

The calendar is for the Fall semester class, 2008.

Day:Date Content
8/25 Introduction, Chemistry basics, Mole concept and calculations (Lecture), Laboratory familiarization
9/1

Labor Day Holiday

9/8

Amino acids, pK values, ionization, dissociation and association constants, acid-base balance

Laboratory #1: Dissociation Constants and pK values for metabolites of the phosphagen and glycolytic-lactate energy systems

9/15

Introduction to Phlebotomy and Occupational Safety and Health (EPL)

Administration (OSHA) Guidelines for handling human body fluids (EPL)

Learning Phlebotomy (EPL)

9/22 Blood sampling, Blood processing, Centrifugation, Refrigeration and Freezing (Lab)

Pippeting (Lab)

Hand in lab 1

9/29

Spectrophotometry, Beers Law, and equipment familiarization (Lecture)

Preparation for Laboratory 2

10/6 Blood collection and processing techniques
10/13

Introduction to Laboratory #3: Hematology - Effects of incremental exercise on hematocrit, hemoglobin and D plasma volume 

10/20

Laboratory #4: Serum Protein (Biuret method) and Lactate Threshold Testing

Hand in lab 2

10/27

Laboratory #5: Oral Glucose Tolerance Test Responses To ...................... (devise own experiment)

Overview of glucose assay

11/3

Laboratory #3, 4 and 5 continued

Hand in lab 3

11/10

Laboratory #5 continued

Hand in lab 4

11/17

Catch-up with Dr. Robergs

11/24 Enzyme Kinetics (Lecture)

Enzyme Kinetics (Lab demonstration)

Laboratory #6, Enzyme Kinetics, Hand in lab 5

12/1 Laboratory #6, cont'd.
12/8

Review for final

Hand in lab 6

12/15

Final Exam 5:30-7:30pm, Teaching Lab