Course Outline
Lecture Notes
Readings
Assay Manual
Experiments
Assays | |
The calendar is for the Fall semester class,
2010.
| 8/23 |
Introduction, Chemistry
basics, Mole concept
and calculations (Lecture), Laboratory familiarization
Colorimetry, Spectrophotometry, Beers
Law, and equipment familiarization (Lecture)
|
| 8/30 |
Introduction to Phlebotomy and Occupational Safety and
Health Administration (OSHA) Guidelines for handling human
body fluids (EPL)
Learning Phlebotomy and intra-venous catheterization (EPL)
Laboratory #1: Pippeting, Buffers, Dilutions, Standard curves,
and Spectrophotometry
|
| 9/6 |
Labor Day Holiday
|
| 9/13 |
Blood sampling, Blood
processing, Centrifugation, Refrigeration and Freezing (Lab);
Spectrophotometry
Introduction to Laboratory
#2: Hematology - Effects of
incremental exercise on
hematocrit, hemoglobin and D
plasma volume
Introduction to Laboratory #3: Serum Protein (Biuret method)
Hand in lab 1 |
| 9/20 |
Dr. Robergs in Australia; Laboratory #2 and 3, cont'd |
| 9/27 |
Dr.
Robergs in Australia; Laboratory #2 and 3, cont'd |
| 10/4 |
Laboratory #4: Lactate Threshold Testing
Overview of the lactate assay
|
| 10/11 |
Laboratory #4, cont'd
Hand in lab 2
|
| 10/18 |
Hand in lab 3
|
| 10/25 |
Laboratory #5: Oral Glucose Tolerance Test Responses To
...................... (devise own experiment)
Overview of glucose assay
Hand
in lab 4 |
| 11/1 |
Laboratory #5, cont'd
|
| 11/8 |
Amino acids, pK values,
ionization, dissociation and association constants, acid-base balance and
buffers
Enzyme Kinetics |
| 11/15 |
Enzyme Kinetics (Lab demonstration)
Laboratory #6,
Enzyme Kinetics, Hand in lab 5
|
| 11/22 |
Laboratory #6, cont'd. |
| 11/29 |
Lets Make a Sports Drink! |
| 12/6 |
Group Oral Reports: Sports Drink Taste Testing and Ingredient List,
Concentrations and Rationale
Course overview and evaluations
Hand in lab 6 |
|
