Assay Manual

PEP 426
PEP 593-EEE
PEP 535-EB
Biochem Lab
PEP 593
PEP 627
PEP 528-Neuromuscular Perf.
PEP/HED604
530 Lab. Proced.
Research Methods
PEP 478/579
LabVIEW
PEP 326

The manual you are to complete requires descriptions of 10 metabolite assays. Preferably, the assays should suite spectrophotometer or fluorometer use. However, if you have an interest in a molecule that involves a more elaborate assay procedure (eg. radioimmuno-assay, double antibody, ELISA, etc.) you can also include these.

If you decide to choose metabolites or hormones, etc. that do not require enzymatic spectrophotometry that is fine, but you are only allowed to include 5 of these. Thus, I still want you to have at least 5 metabolites that involve enzymatic spectrophotometry/fluorometry!

I recommend that you consult either or both of the following references:

Methods in Enzymology, QU 25 M56 1955- UNM Medical Library

Bergmeyer J. and M. Grassl. Methods of Enzymatic Analysis., QU 25 B45 1983-'89, UNM Medical Library

You are expected to include the following in each of your assay descriptions:

	A brief background of the metabolism/biochemistry that concerns this metabolite.
	A description of the sample conditions necessary for the eventual measurement of this metabolite.
	The reactions involved in the assay.
	A table of the cocktail ingredients, a normal stock solution of each, the desired final concentration in an appropriate volume of cocktail, and the volume of stock needed to get the desired concentration. See my examples for the glucose and glycerol assays.
	Additional references supporting this assay, some identifying other forms of the assay, and research articles that reference this assay in Methods.
	Anything else that you may feel will help you use this information in the future.