Research Publications (1991-2000):  

43. Novel Enzymic Hydrolytic Dehalogenation of a Chlorinated Aromatic. J. D. Scholten, K.-H. Chang P. C. Babbitt, H. Charest, M. Sylvestre and D. Dunaway-Mariano*, Science 253: 182 (1991).

44. Evidence for an Intra Molecular, Stepwise Reaction Pathway for PEP Phosphomutase Catalyzed P-C Bond Formation. M. S. McQueney, S.-L. Lee, W H. Swartz, H. L. Ammon, P. S. Mariano and D. Dunaway-Mariano*, J. Org. Chem. 56: 7121 (1991).

45. Expression of the 4-Chlorobenzoate Dehalogenase Genes From Pseudomonas sp. CBS-3 in Escherichia coli and Identification of the Gene Translation Products. P. Savard, H. Charest, F. Schareck, M. Sylvestre, J. D. Scholten and D. Dunaway-Mariano*, Canadian J. Microbiol. 38: 1074 (1992).

46. Investigation of the Substrate Binding and Catalytic Groups of the P-C Bond Cleaving Enzyme Phosphonoacetaldehyde Hydrolase, D. B. Olsen, T. W. Hepburn, S.-L. Lee, B. M. Martin, P. S. Mariano and Debra Dunaway-Mariano*, Archives of Biochemistry & Biophysics 296: 144 (1992).

47. Isolation and Characterization of the Three Polypeptide Components of 4-Chlorobenzoate Dehalogenase from Pseudomonas sp. Strain CBS-3. K.-H. Chang, P.-H. Liang, W. Beck, J. D. Scholten and D. Dunaway-Mariano*, Biochemistry 31: 5605 (1992).

48. Ancestry of the 4-Chlorobenzoate Dehalogenase: Analysis of Amino Acid Sequence Identities Among Families of Acyl: Adenyl Ligases, Enoyl-CoA Hydratases/Isomerases and Acyl-CoA Thioesterases. P. C. Babbitt*, G. L. Kenyon, B. M. Martin, H. Charest, M. Sylvestre, J. D. Scholten, K.-H. Chang, P.-H Liang and D. Dunaway-Mariano*, Biochemistry 31: 5594 (1992).

49. A Stereochemical Probe for the Mechanism of P-C Bond Cleavage Catalyzed by the B. cereus Phosphonoacetaldehyde Hydrolase. S.-L. Lee, T. W. Hepburn, W. H. Swartz, P. S. Mariano* and D. Dunaway-Mariano*, J. Am. Chem. Soc. 114: 7346 (1992).

50. Characterization of the Covalent Enzyme Intermediates Formed During Pyruvate Phosphate Dikinase Catalysis. S. H. Thrall, A. F. Mehl, L. J. Carroll and D. Dunaway-Mariano*, Biochemistry. 32: 1803 (1993).

51. Investigations of Kinase Substrate Specificity with Aqua Rh(III) Complexes of Adenosine 5'-Triphosphate, Z. Lu, A.L. Shorter and D. Dunaway-Mariano*, Biochemistry. 32: 2378 (1993).

52. Specificity of 4-Chlorobenzoate-Coenzyme A Dehalogenase Catalyzed Dehalogenation of Halogenated Aromatics. P.-H. Liang, G. Yang and D. Dunaway-Mariano*, Biochemistry 32: 12245 (1993).

53. Kinetic Evidence for Separate Site Catalysis by Pyruvate Phosphate Dikinase, S.A. Thrall and D. Dunaway-Mariano, (1994) Biochemistry 33: 1103.

54. The Energetics Pyruvate Phosphate Dikinase Catalysis. A. Mehl, Y. Xu and D. Dunaway-Mariano*, (1994) Biochemistry 33: 1093.

55. Substrate Binding Domains in Pyruvate Phosphate Dikinase. L.J. Carroll, Y. Xu, S.H. Thrall, B. M. Martin and D. Dunaway-Mariano*, (1994) Biochemistry 33: 1134.

56. On the Origin and Mechanism of 4-Chlorobenzoyl-CoA Dehalogenase. Debra Dunaway-Mariano* and Patricia Babbitt, (1994) Biodegradation, 5, 0000.

57. Evidence for Nucleophilic Catalysis in the Aromatic Substitution Reaction Catalyzed by (4-Chlorobenzoyl)enzyme A Dehalogenase, G. Yang, P.-H. Liang and D. Dunaway-Mariano (1994) Biochemistry 33: 8527.

58. Separate Site Catalysis by Pyruvate Phosphate Dikinase as Revealed by Deletion Mutants, Y. Xu, M. McGuire, B.M. Martin and D. Dunaway-Mariano, (1995) Biochemistry 34: 2195-2202.

59. Location of the Catalytic Site for PEP Formation within the Primary Structure of Clostridium Symbiosum Pyruvate Phosphate Dikinase I. Identification of an Essential Cysteine by Chemical Modification with [1-14C]Bromopyruvate and Site Directed Mutagenesis. X. Yu, S. Li, L. Yankie, Y.-S. Jung, P.S. Mariano, B.M. Martin and D. Dunaway-Mariano, (1995) Biochemistry 34: 2181-2187.

60. Location of the Catalytic Site for PEP Formation within to Primary Structures of Clostridium Symbiosum Pyruvate Phosphate Dikinase II. Site Directed Mutagenesis of an Essential Arginine. L. Yankie, Y. Xu and Debra Dunaway-Mariano, (1995) Biochemistry 34: 2188-2194.

61. Transient State Kinetic Analysis of the Chemical Intermediates of the 4-Chlorobenzoyl-Coenzyme A Dehalogenase Catalyzed Aromatic Substitution Reaction. R.Q. Liu, P.O. Liang, J. Scholten and D. Dunaway-Mariano (1995) J. Am. Chem. Soc., 117: 5003.

62. Evidence for Electrophilic Catalysis in the 4-Chlorobenzoyl-CoA Dehalogenase Reaction: UV, Raman, and 13C-NMR Spectral Studies of Dehalogenase Complexes of Benzoyl-CoA Adducts. K. Taylor, R-Q. Liu, P.-H. Liang, J. Price, D. Dunaway-Mariano, P.J. Tonge, J. Clarkson and P.R. Carey. (1995) Biochemistry 34: 13881-13888.

63. Swiveling-Domain Mechanism for Enzymatic Phosphotransfer Between Remote Reaction Sites. O. Herzberg, C.C.H. Chen, G. Kapadia, M. McGuire, L.J. Carroll, S. J. Noh and Debra Dunaway-Mariano (1996) Proc. Nat. Acad. Sci. USA, 93: 2652-2657.

64. Phosphoenolpyruvate Mutase Catalysis of Phosphoryl Transfer in Phosphoenol Pyruvate: Kinetics and Mechanism of Phosphorus-Carbon Bond Formation. J. Kim and D. Dunaway-Mariano (1996) Biochemistry 35: 4628-4635.

65. Structure of 4-Chlorobenzoyl Coenzyme A Dehalogenase Determined to 1.8 A Resolution: An Enzyme Catalyst Generated via Adaptive Mutation. M. M. Benning, K. L. Taylor, R.-Q. Liu, G. Yang, H. Xiang, G. Wesenberg, D. Dunaway-Mariano and H. Holden (1996) Biochemistry 35: 8103-8109.

66. Determination of the Nucleotide Binding Sites within Clostridium Symbiosum Pyruvate Phosphate Dikinase by Photoaffinity Labeling, Site-Directed Mutagenesis and Structural Analysis. M. McGuire, L.J. Carroll, L. Yankie,, S. Thrall, D. Dunaway-Mariano, O. Herzberg, B. Jayaram and B. Haley (1996) Biochemistry 35: 8544-8552.

67. Identification of Active Site Residues Essential to 4-Chlorobenzoyl-Coenzyme A Catalysis by Chemical Modification and Site Directed Mutagenesis.Yang, G., Taylor, K. L., Liu, R.-Q., Xiang, H., Price, J. and Dunaway-Mariano, D. (1996) Biochemistry 35: 10879-10885. [supporting material]

68. Determination of the Chemical Pathway for 4-Chlorobenzoate: Coenzyme A Ligase Catalysis. Chang, K.-H. and Dunaway-Mariano, D. (1996) Biochemistry 35: 13478-13484.[supporting material]

69. Investigation of Substrate Activation by 4-Chlorobenzoyl Coenzyme Dehalogenase.Taylor, K. L , Xiang, H., Liu, R.-Q.and Dunaway-Mariano, D. (1997) Biochemistry 36: 1349-1361.

70. Studies on the Structure, Mechanism of Catalysis and Ancestry of 4-Chlorobenzoyl-Coenzyme A Dehalogenase. Dunaway-Mariano, D. (1997) Proced. Nat. Acad. Sci. Netherlands, 143-156.

71. Raman-Study of the Polarizing Forces Promoting Catalysis in 4-Chlorobenzoate-CoA Dehalogenase Clarkson J, Tonge P. J., Taylor K. L., Dunaway-Mariano D., Carey P. R. (1997) Biochemistry 36: 10192-10199.

72. Acyl-Adenylate Motif of the Acyl-Adenylate/Thioester-Forming Enzyme Superfamily : A Site-Directed Mutagenesis Study with the Pseudomonas Sp. Strain Cbs3 4-Chlorobenzoate-Coenzyme-A Ligase. Chang K. H., Xiang H., Dunaway-Mariano D. (1997) Biochemistry 36:15650-15659.

73 . Studies on the structure, catalytic mechanism and ancestry of the 4-chlorobenzoate-coenzyme A dehalogenase.    Verhandelingen - Koninklijke Nederlandse Akademie van Wetenschappen, Afdeling Natuurkunde,   Dunaway-Mariano, D..  (1997) Tweede Reeks , 98(Mechanisms of Biohalogenation and Dehalogenation),  143-156.

74. Isolation and Characterization ot the Carbon-Phosphorus Bond-Forming Enzyme Phosphoenoylpyruvate Mutase from the MOLLUSK MYTILUS-EDULISAlexander Kim, Jaebong Kim, Brian M. Martin and Debra Dunaway-Mariano. (1998) J. Biol. Chem. , v. 273(#8): 4443-4448

75. Insights Into the Mechanism of Catalysis by the P-C Bond Cleaving Enzyme Phosphonoacetaldehyde Hydrolase Derived from Gene Sequence-Analysis and Mutagenesis. Baker, A.S., Ciocci, M. J., Metcalf, W.W., Kim, J.-B., Babbitt, P. C., Wanner, B. L., Martin, B. M., Dunaway-Mariano, D (1998) Biochemistry 37: 9305-9315.

76. Location of the Phosphate Binding Site within Clostridium Symbiosum Pyruvate Phosphate Dikinase M.McGuire, K. Haung, G. Kapadia, O. Herzberg and D. Dunaway-Mariano (1998) Biochemistry37: 13463-1374.[Supporting Material]

77. The Three-dimensional Structure of 4-Hydroxybenzoyl-CoA Thioesterase from Pseudomonas s. strain CBS-3. M. M. Benning, G. Wesenberg, R. Liu, K. L. Taylor, D. Dunaway-Mariano and H. Holden (1998) J. Biol. Chem. 273: 33572-33579.

78. Intermediates and energetics in Pyruvate Phosphate Dikinase. (1999)Dunaway-Mariano Methods Enzymology, 308:149-176.

79. The Template Mechanism of Enzyme Evolution. Xiang, H., Taylor, K. and Dunaway-Mariano, D. (1999) Biomedical Health Research vol 27, Ed. P. Frey & D. Northrop, IOS Press, Washington D.C.224-234.

80. Product Catalyzes the Deamidation of D154N Dehalogenase to Produce the Wild-Type Enzyme H. Xiang, J. Dong, P. R. Carey and D. Dunaway-Mariano (1999) Biochemistry 38: 4207-4213.[Supporting Material]

81. Modulating the Elecron Density in the Bound Product, 4-Hydroxybenzoyl-CoA, By Mutations in the 4-Chlorobenzoyl-CoA Dehalogenase Near the 4-Hydroxy-Group. J. Dong, H. Xiang, L. Luo, Debra Dunaway-Mariano and Paul Carey (1999) Biochemistry 38: 4198-4206.[Supporting Material]

82. Helix swapping between two aa/bbbarrels: crystal structure of phosphoenolpyruvate mutase with bound Mg 2+ oxalate K. Huang, Z. Li, Y. Jia, Dunaway-Mariano, D and O. Herzberg, (1999) Structure 7: 539-548.

83. Interchange of Catalytic Activity within the2-Enoyl-Coenzyme A Hydratase/Isomerase Superfamily Based on a Common Active Site Template H. Xiang, L. Luo, K. L. Taylor, and D. Dunaway-Mariano (1999) Biochemistry 38: 7638-7652

84. Insight into the mechanism of phosphoenolpyruvate mutase catalysis derived from site-directed mutagenesis studies of active site residues. Y. Jia, Z. Lu, K. Huang, O. Herzberg, and D. Dunaway-mariano (1999) Biochemistry 38: 14165-14173.

85. Crystallization and preliminary crystallographic analysis of phosphonoacetaldehyde hydrolase. M.C.Morais, A.S. Baker, D.Dunaway-Mariano, and K.N.Allen (2000) Acta Crytallogr. D.Biol.Crystallogr. 56 (Pt 2): 206-209.

86. The crystal structure of Bacillus cereus phosphonoacetaldehyde hydrolase: insight into catalysis of phosphorus bond cleavage and catalytic diversification within the HAD enzyme superfamily.M.C. Morais, W.Zhang, A.S.Baker, G.Zhang, D. Dunaway-Mariano, and K.N. Allen. (2000) Biochemistry 39: 10385-10396.

87. Identification of Domain-Domain Docking Sites within Clostridium symbiosum Pyruvate Phosphate Dikinase by Amino Acid Replacement. M. Wei, Z. Li, D. Ye, O. Herzberg, and D. Dunaway-Mariano. (2000) J. Biol. Chem. 275: 41156-41165.

88. Raman difference spectroscopic studies of dithiobenzoyl substrate and product analogs binding to the enzyme dehologenase: pi-electron polarization is prevented by C=O to C=S substitution. J. Dong, L. Luo, P. Liang, D. Dunaway-Mariano and P. Carey (2000) J. Raman Spectroscopy , 31:365-371.

For the research publications after 2000, please click this link: 2001-2005.